Pokusna kokcidioza uzrokovana vrstom Eimeria adenoeides u purića koji su dobivali ohratoksin A.

The progression of coccidiosis provoked by E. adenoeides was followed in turkey poults given ochratoxin A (OTA) in feed. More heavy and rapid progress of coccidiosis occurred in OTA-treated turkey poults than in those fed an OTA-free diet, as can be seen from the higher levels of oocyst and lesion indices and more rapid mortality in such birds. The concentration of total protein in serum was significantly increased in turkey poults infected with E. adenoeides and simultaneously given OTA. The serum concentration of uric acid was significantly increased in all experimental groups, especially in those exposed to OTA, and most notably in the group additionally infected with E. adenoeides. OTA induced degenerative changes in kidneys, liver and heart as well as depletion of lymphoid tissue in the lymphoid organs and a decrease in body mass. Coccidiosis also induced a growth depression in addition to caecal haemorrhages. Impairment of kidney function, histopathological changes and general growth depression were stronger when turkey poults infected with E. adenoeides were also given OTA.Istraživan je tijek kokcidioze uzrokovan protozoonom E. adenoeides u purića koji su dobivali ohratoksin u hrani. Znatno teži oblik bolesti uočen je u purića pokusne u odnosu na kontrolnu skupinu. Razlika je uočena na razini broja oocista, stupnja oštećenja i bržeg uginuća. Koncentracija ukupnih proteina u serumu bila je također značajno povišena u pokusne skupine. Koncentracija mokraćne kiseline u serumu bila je osobito povišena uskupini invadiranoj kokcidijama i hranjenoj hranom s primiješanim ohratoksinom. Ohratoksin je uzrokovao degenerativne promjene u bubrezima, jetrima i srcu kao i depleciju limfoidnog tkiva te smanjenje tjelesne težine. Kokcidioza je također uzrokovala smanjeni rast, ali i krvarenje u slijepim crijevima. Patohistološke promjene, smanjena funkcija bubrega kao i opće smanjen prirast bili su izraženiji u invadirane skupine koja je u hrani dobivala ohratoksin

A chromosomal analysis of Nepa cinerea Linnaeus, 1758 and Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepidae)

An account is given of the karyotypes and male meiosis of the Water Scorpion Nepa cinerea Linnaeus, 1758 and the Water Stick Insect Ranatra linearis (Linnaeus, 1758) (Heteroptera, Nepomorpha, Nepidae). A number of different approaches and techniques were tried: the employment of both male and female gonads and mid-guts as the sources of chromosomes, squash and air-drying methods for chromosome preparations, C-banding and fluorescence in situ hybridization (FISH) for chromosome study. We found that N. cinerea had a karyotype comprising 14 pairs of autosomes and a multiple sex chromosome system, which is X1X2X3X4Y (♂) / X1X1X2X2X3X3X4X4 (♀), whereas R. linearis had a karyotype comprising 19 pairs of autosomes and a multiple sex chromosome system X1X2X3X4Y (♂) / X1X1X2X2X3X3X4X4 (♀). In both N. cinerea and R. linearis, the autosomes formed chiasmate bivalents in spermatogenesis, and the sex chromosome univalents divided during the first meiotic division and segregated during the second one suggesting thus a post-reductional type of behaviour. These results confirm and amplify those of Steopoe (1925, 1927, 1931, 1932) but are inconsistent with those of other researchers. C-banding appeared helpful in pairing up the autosomes for karyotype assembly; however in R. linearis the chromosomes were much more uniform in size and general appearance than in N. cinerea. FISH for 18S ribosomal DNA (major rDNA) revealed hybridization signals on two of the five sex chromosomes in N. cinerea. In R. linearis, rDNA location was less obvious than in N. cinerea; however it is suggested to be similar. We have detected the presence of the canonical “insect” (TTAGG)n telomeric repeat in chromosomes of these species. This is the first application of C-banding and FISH in the family Nepidae.Copyright Robert B. Angus et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.NHM Repositor

Morphological variability of the Bulgarian endemic Betonica bulgarica Degen et Neič. (Lamiaceae) from Sinite Kamani Natural Park, Eastern Balkan Range

Four populations of Betonica bulgarica Degen et Neič. at Sinite Kamani Natural Park were morphologically tested. Intrapopulation and interpopulation variabilities were established. The rеlationship between morphological variability, number, area and ecological appurtenance of the studied populations were explored. The results demonstrated that the main source of phenotype variation is intrapopulation variability, mainly due to the age structure of populations. The most variable traits are height of stem and dimensions of leaves. The registered interpopulation variability was affected by the differences in altitude, soil type and differences in environmental conditions and soil properties. Indumentum and morphology of generative organs had taxonomic signifi cance for distinguishing B. bulgarica from the other species in the genus, including the species that were morphologically most similar to it – Betonica officinalis L

Genome-wide linkage analysis of 972 bipolar pedigrees using single-nucleotide polymorphisms.

Because of the high costs associated with ascertainment of families, most linkage studies of Bipolar I disorder (BPI) have used relatively small samples. Moreover, the genetic information content reported in most studies has been less than 0.6. Although microsatellite markers spaced every 10 cM typically extract most of the genetic information content for larger multiplex families, they can be less informative for smaller pedigrees especially for affected sib pair kindreds. For these reasons we collaborated to pool family resources and carried out higher density genotyping. Approximately 1100 pedigrees of European ancestry were initially selected for study and were genotyped by the Center for Inherited Disease Research using the Illumina Linkage Panel 12 set of 6090 single-nucleotide polymorphisms. Of the ~1100 families, 972 were informative for further analyses, and mean information content was 0.86 after pruning for linkage disequilibrium. The 972 kindreds include 2284 cases of BPI disorder, 498 individuals with bipolar II disorder (BPII) and 702 subjects with recurrent major depression. Three affection status models (ASMs) were considered: ASM1 (BPI and schizoaffective disorder, BP cases (SABP) only), ASM2 (ASM1 cases plus BPII) and ASM3 (ASM2 cases plus recurrent major depression). Both parametric and non-parametric linkage methods were carried out. The strongest findings occurred at 6q21 (non-parametric pairs LOD 3.4 for rs1046943 at 119 cM) and 9q21 (non-parametric pairs logarithm of odds (LOD) 3.4 for rs722642 at 78 cM) using only BPI and schizoaffective (SA), BP cases. Both results met genome-wide significant criteria, although neither was significant after correction for multiple analyses. We also inspected parametric scores for the larger multiplex families to identify possible rare susceptibility loci. In this analysis, we observed 59 parametric LODs of 2 or greater, many of which are likely to be close to maximum possible scores. Although some linkage findings may be false positives, the results could help prioritize the search for rare variants using whole exome or genome sequencing

Pattern electroretinography as an objective method for study the visual analyzer function in patients with diabetes mellitus with initial diabetic retinopathy

Цел: Целта на нашето проучване е да се изследва обективно функцията на зрителния анализатор чрез патерна електроретинография (ПЕРГ) при пациенти със захарен диабет (ЗД) с начална диабетна ретинопатия (ДР) и получените резултати да се сравнят с контролната група, както и според типа на диабета.Материал и методика: Изследвана е група от 81 човека (162 очи), от които 47 здрави лица (контроли) - 94 очи. Пациентите със ЗД са 34 (68 очи), от които със ЗД тип 1 са 11 лица и 23 са с тип 2 ЗД. Извършена е ПЕРГ. Основните показатели, които са отчетени при анализа на резултатите са латентни времена и амплитуди, отразяващи се на конфиrурацията на вълновите форми.Резултати: При сравнителния анализ на стойностите на компонентите на ПЕРГ с контролната група, както и според типа ЗД, се установиха значителни различия. При сравнителния анализ на стойностите на компонентите на ПЕРГ между пациенти със ЗД с ДР и контроли се установи, че двете групи статистически се различават по амплитудите на всички компоненти на ПЕРГ при всички отвеждания при 15o и 30o.При латентностите сигнификантни различия се установиха при компонент Р50 при всички отвеждания при 15o и 30o. Пациентите със ЗД имат сигнификантно по-удължени латентности и по-ниски амплитуди, в сравнение с контролната група. При сравнителния анализ според типа ЗД, сигнификантни различия се получиха при амплитуден компонент P50-N95 при 15o и30o при дясно око, което потвърждава асиметричното засягане на очите при ЗД. Амплитудите на ПЕРГ при ЗД тип 2 са сигнификантно по-ниски от тези на пациентите със ЗД тип 1.Заключение: ПЕРГ би могла да се използва като обективен метод за регистриране на ранни изменения във функцията на зрителния анализатор (ЗА) като усложнение на З)J,. Също така и за проследяване на промените в динамика, тъй като изследването е неинвазивно, безвредно, по-бързо, повторяемо и обективно, по-евтино в сравнение с флуоресцеиновата ангиография (ФА), оптината кохерентна томография (ОСТ) и ангио-ОСТ.Aim: The aim of the study was to examine objectively the visual analyzer function by pattern electroretinography (PERG) in patients with diabetes mellitus (DM) with initial diabetic retinopathy (DR) and to compare the results with the control group as well as according to the type of diabetes. Material and methods: A group of 81 people (162 eyes) were studied. Patients with DM were 34 (68 eyes), 11 patients with type 1 DM and 23 with type 2 DM. The control group consisted of 47 healthy individuals (94 eyes). PERG was performed. The main variables that were considered in the results analysis were the latency and amplitudes, reflecting the configuration of the wave forms.Results: The comparative analysis of PERG components between patients with DM with DR and controls as well as according to the type of DM demonstrated significant differences. In latencies, significant differences were found for component P50 at all electrode positions at 15o and 30o. Patients with DM had significantly longer latencies and lower amplitudes compared to the control group. In the comparative analysis according to the type of DM, significant differences were found in amplitude component P50-N95 at 15o and 30o in the right eye, which confirms the asymmetrical eye involvement in DM. The PERG amplitudes in type 2 DM were significantly lower than those of type 1 DM patients.Conclusion: PERG could be used as an objective method for registration of early changes in the visual analyzer function as a DM complication. Also, to monitor the changes in dynamics as it is non-invasive, harmless, faster, and less expensive than fluorescein angiography (FA), OCT and angio-OCT

Identification of tomato accessions as source of new genes for improving heat tolerance: from controlled experiments to field

Background: Due to global warming, the search for new sources for heat tolerance and the identification of genes involved in this process has become an important challenge as of today. The main objective of the current research was to verify whether the heat tolerance determined in controlled greenhouse experiments could be a good predictor of the agronomic performance in field cultivation under climatic high temperature stress. Results: Tomato accessions were grown in greenhouse under three temperature regimes: control (T1), moderate (T2) and extreme heat stress (T3). Reproductive traits (flower and fruit number and fruit set) were used to define heat tolerance. In a first screening, heat tolerance was evaluated in 219 tomato accessions. A total of 51 accessions were identified as being potentially heat tolerant. Among those, 28 accessions, together with 10 accessions from Italy (7) and Bulgaria (3), selected for their heat tolerance in the field in parallel experiments, were re-evaluated at three temperature treatments. Sixteen tomato accessions showed a significant heat tolerance at T3, including five wild species, two traditional cultivars and four commercial varieties, one accession from Bulgaria and four from Italy. The 15 most promising accessions for heat tolerance were assayed in field trials in Italy and Bulgaria, confirming the good performance of most of them at high temperatures. Finally, a differential gene expression analysis in pre-anthesis (ovary) and post-anthesis (developing fruit) under heat stress among pairs of contrasting genotypes (tolerant and sensitive from traditional and modern groups) showed that the major differential responses were produced in post-anthesis fruit. The response of the sensitive genotypes included the induction of HSP genes, whereas the tolerant genotype response included the induction of genes involved in the regulation of hormones or enzymes such as abscisic acid and transferases. Conclusions: The high temperature tolerance of fifteen tomato accessions observed in controlled greenhouse experiments were confirmed in agronomic field experiments providing new sources of heat tolerance that could be incorporated into breeding programs. A DEG analysis showed the complex response of tomato to heat and deciphered the different mechanisms activated in sensitive and tolerant tomato accessions under heat stress

Identification of tomato accessions as source of new genes for improving heat tolerance: from controlled experiments to field

Background: Due to global warming, the search for new sources for heat tolerance and the identification of genes involved in this process has become an important challenge as of today. The main objective of the current research was to verify whether the heat tolerance determined in controlled greenhouse experiments could be a good predictor of the agronomic performance in field cultivation under climatic high temperature stress. Results: Tomato accessions were grown in greenhouse under three temperature regimes: control (T1), moderate (T2) and extreme heat stress (T3). Reproductive traits (flower and fruit number and fruit set) were used to define heat tolerance. In a first screening, heat tolerance was evaluated in 219 tomato accessions. A total of 51 accessions were identified as being potentially heat tolerant. Among those, 28 accessions, together with 10 accessions from Italy (7) and Bulgaria (3), selected for their heat tolerance in the field in parallel experiments, were re-evaluated at three temperature treatments. Sixteen tomato accessions showed a significant heat tolerance at T3, including five wild species, two traditional cultivars and four commercial varieties, one accession from Bulgaria and four from Italy. The 15 most promising accessions for heat tolerance were assayed in field trials in Italy and Bulgaria, confirming the good performance of most of them at high temperatures. Finally, a differential gene expression analysis in pre-anthesis (ovary) and post-anthesis (developing fruit) under heat stress among pairs of contrasting genotypes (tolerant and sensitive from traditional and modern groups) showed that the major differential responses were produced in post-anthesis fruit. The response of the sensitive genotypes included the induction of HSP genes, whereas the tolerant genotype response included the induction of genes involved in the regulation of hormones or enzymes such as abscisic acid and transferases. Conclusions: The high temperature tolerance of fifteen tomato accessions observed in controlled greenhouse experiments were confirmed in agronomic field experiments providing new sources of heat tolerance that could be incorporated into breeding programs. A DEG analysis showed the complex response of tomato to heat and deciphered the different mechanisms activated in sensitive and tolerant tomato accessions under heat stress

Copy number variation in bipolar disorder.

Large (>100 kb), rare (500 kb) CNVs in BD compared with SZ, most notably for deletions >1 Mb (P=9 × 10(-4))

Phenotypic insights into ADCY5-associated disease

BACKGROUND: Adenylyl cyclase 5 (ADCY5) mutations is associated with heterogenous syndromes: familial dyskinesia and facial myokymia; paroxysmal chorea and dystonia; autosomal-dominant chorea and dystonia; and benign hereditary chorea. We provide detailed clinical data on 7 patients from six new kindreds with mutations in the ADCY5 gene, in order to expand and define the phenotypic spectrum of ADCY5 mutations. METHODS: In 5 of the 7 patients, followed over a period of 9 to 32 years, ADCY5 was sequenced by Sanger sequencing. The other 2 unrelated patients participated in studies for undiagnosed pediatric hyperkinetic movement disorders and underwent whole-exome sequencing. RESULTS: Five patients had the previously reported p.R418W ADCY5 mutation; we also identified two novel mutations at p.R418G and p.R418Q. All patients presented with motor milestone delay, infantile-onset action-induced generalized choreoathetosis, dystonia, or myoclonus, with episodic exacerbations during drowsiness being a characteristic feature. Axial hypotonia, impaired upward saccades, and intellectual disability were variable features. The p.R418G and p.R418Q mutation patients had a milder phenotype. Six of seven patients had mild functional gain with clonazepam or clobazam. One patient had bilateral globus pallidal DBS at the age of 33 with marked reduction in dyskinesia, which resulted in mild functional improvement. CONCLUSION: We further delineate the clinical features of ADCY5 gene mutations and illustrate its wide phenotypic expression. We describe mild improvement after treatment with clonazepam, clobazam, and bilateral pallidal DBS. ADCY5-associated dyskinesia may be under-recognized, and its diagnosis has important prognostic, genetic, and therapeutic implications. © 2016 The Authors. Movement Disorders published by Wiley Periodicals, Inc. on behalf of International Parkinson and Movement Disorder Society